DEPARTMENT OF MICROBIOLOGY


Faculty


  • Dr. V.Balaji, MD, Ph.D - Professor
  • Dr. Joy S Michael, MD, FRCPath - Professor
  • Dr. John Antony Jude Prakash, MD - Professor
  • Dr. Shalini Anandan, MD - Associate Professor
  • Dr. Rani Diana Sahni, MD - Assistant Professor
  • Dr. Shakti Laishram, MD – Assistant Professor

Academic Microbiology Provision

The Department of Microbiology runs the following courses:

  • MD (Microbiology) – 3 year
  • MSc (Medical Microbiology) – 3 year
  • PG Diploma in Medical Microbiology – 1 year plus 1 year internship

The faculty and staff are also involved in the teaching of the following courses:

Undergraduate courses

  • MBBS
  • BSc MLT
  • BPT/BOT
  • BSc Nursing
  • PC BSc Nursing
  • Diploma in Nursing
  • BMRSc
  • Anaesthesia Technology
  • Respiratory Technology
  • Perfusion Technology

Postgraduate courses

  • MD Microbiology
  • MSc Medical Microbiology
  • PG Dip Medical Microbiology
  • Diploma in Clinical Pathology
  • Physician Nurse Assistant

Microbiology postings are held for the following courses:

  • MD Dermatology
  • MS Ophthalmology
  • MS ENT
  • MS General Surgery
  • MPhil (Hospital Administration)

Other courses

  • Integrated Post Graduate Diploma in Family Medicine (PGDFM)
  • Tropical Medicine Course (IDTRC)

Laboratory Microbiology Provision

The department offers a 24 hour microbiology laboratory service to the entire hospital, its outreach programs, peripheral hospitals and sister institutions. The services are divided into sections, with a faculty member overseeing and authorizing the work in each section.

Tests performed in Microbiology

A). Bacteriology:

Services offered:Culture, identification of pathogens, Antibiotic Susceptibility Testing (AST) of isolates

Specimens tested:

- Blood & bone marrow - Automated BacT Alert system

- CSF and other body fluids

- Tissue/Biopsy material

- Exudates

- Catheters

- Faeces(Dark field Microscopy for V cholerae)

- Urine Culture includes Supra-pubic aspirate / catheters, Cystoscopy specimen & Percutaneous nephrostomy (PCN), ileal conduct / clean intermittent catheter samples

- Sputum. Bronchoalveolar lavage (BAL), Endotracheal aspirates (ETA)

- Throat, Nasal and Ear Swabs

- Genital swabs


Antimicrobial Susceptibility Testing

- Disc diffusion

- MIC determination

Anaerobic Culture

- Microscopy, culture and AST

B). Mycology:

- Microscopy & Culture

- Cryptococcal antigen on CSF

Antifungal Susceptibility Testing

- Bronchial wash or induced sputum for Pneumocystis jirovecii –stain direct fluorescence antibody detection

C). Mycobacteriology:

- Smear -fluorescence microscopy

- Culture – solid and automated liquid culture (MGIT)

- Drug susceptibility testing (by solid & MGIT)

- TB PCR

- QuantiFERON TB Gold

D). Serology:

Febrile agglutination tests

Widal, Weil Felix, Brucella, Mycoplasma Cold Agglutination

VDRL

TPPA

Nephelometer

C3, C4, CRP, ASO, ADNB, RA

Total complement

Autoimmune serology

Antinuclear antibodies

Anti ds-DNA

Anti- CCP

Scleroderma SCL 70

JO 1

Centrome nucleoprotein (CNP)

Uridyl 1 ribonucleoprotein (U1 RNP)

Anti- Smith SM IgG

Anti- histones IgG

Anti- ribosomal P proteins IgG

Beta 2 Glycoprotein IgG and IgM

Sjogrens syndrome SSA

Sjogrens syndrome SSB

Anti Cardiolipin (ACL)

Myositis profile

Autoimmune hepatitis :

Anti- LKM IgG

Soluble liver & pancreas antigen- SLA/P IGg ELISA & & Liver mosaic IFA

Anti –mitochondrial antibodies

Anti- Thyroid antibodies :

Anti- Throglobulin ELISA &

Anti – Thyroid Peroxidase TPO ELISA

Leptospira IgM

Spotted fever IFA

Scrub typhus IgM

Clostridium difficile toxin detection

E). Surveillance:

Portable water and environmental surveillance, Critical care area surveillance, Microbiology sterility, sterility check of blood bags, solutions and sterilizers

F). Research

  1. WHO sponsored – Invasive Bacterial Disease Surveillance (IBDS) GAVI & PneumoADIP 2006 onwards 2012- The goal of the project is to determine the serotype prevalence of invasive isolates of Streptococcus pneumoniae from India, Sri Lanka and Nepal under the Invasive Bacterial Infectious Surveillance (IBIS ) programme and the Southeast Asian Pneumococcal Network Alliance (SAPNA).
  2. Vaccine preventable - invasive bacterial disease surveillance – Southeast Asia - Sentinel site Co –Investigator & Head microbiology reference center - WHO (SEARO)
  3. Vaccine preventable - invasive bacterial disease surveillance in children, India - Sentinel site Co –Investigator & Head microbiology reference center - ICMR
  4. Neonatal Invasive Bacterial Disease Surveillance- Sentinel site Co –Investigator - ICMR
  5. Asian Network for Surveillance of Resistance System (ANSORP), in India- 2011- Principal investigator- Asia Pacific Foundation for Infectious Disease.
  6. Synbiotics for prevention of necrotizing enterocolitis in India - Principal investigator - DS&T- Gov of India
  7. “Multi Center study on Antimicrobial Resistance, Monitoring of Salmonella Typhi and Salmonella Paratyphi A – An attempt to make national guidelines on antibiotic policy to treat Enteric fever” - Co –Investigator- ICMR
  8. A double blind, randomized, parallel, placebo control design study to determine the effect of addition of vitamin D to conventional anti TB therapy, Dalhousie & ICMR funded project- Since Jan 2010.
  9. Genome sequencing and Genotyping of Mycobacterium tuberculosis isolates from patients with extrapulmonary tuberculosis (EPTB) -Institute of Genomics and Intergrative Biology (IGIB), Delhi. CSIR - Since March 2011
  10. Prevalence and Incidence of tuberculosis infection among Nursing & allied health students in a south Indian teaching hospital, as assessed by QuantiFERON – TB-Gold Interferon assay and Tuberculin Skin test- McGill India Strategic Research Initiative project - Since June 2007
  11. Evaluation of Xpert MTB/Rif assay for the diagnosis of EPTB, CMC Fluid research fund Since Jan 2011.
  12. Fluconazole resistance in patients with esophageal candidiasis and risk factors causing fluconazole resistance. (Sajith KG., Ashok Chako, Mary Mathews, Amith Kumar Dutta, Rani Diana Sahni)
  13. Prevalence of Group B Streptococcal colonization among pregnant women and neonates. ( Sanathanam Sridhar S, Ruby Jose, Rani Diana Sahni, Anil Kurvilla, Niranjan Thomas, Manisha Madhai Beck)
  14. Cohort study of the clinical profile and outcome of patients with urinary tract infection requiring admission into a medical ward in a tertiary care centre in south India. (Divya Bala Thumathy, Rani Diana Sahni, JV Peter, L. Jeyseelan, Thambu David Sudarsanam)
  15. Sero epidemiology of Burkholderia pseudomallei and development of an ELISA for its serodiagnosis Role of Serological and molecular methods in the diagnosis of atypical pneumonia in school age children. Evaluation of Chlamydia trachomatis antigen detection kit .
  16. Chlamydia trachomatis infection in South Indian mothers and transmission to infants. NIH International Clinical Research Fellowship Project.
  17. A comparative study on the prevalence of Chlamydia trachomatis in women with PID suggestive symptoms and those who are asymptomatic.
  18. Study on the Gynaecological Morbidity (including STIs) among married women in the reproductive age group in a tribal population in South India
  19. Phenotypic and genotypic characterization of carbapenem resistance in clinical isolates of Klebsiella pneumonia.
  20. Comparison of effectiveness of povidone-iodine ear drops as compared to 1% clotrimazole + 1% lignocaine ear drops in clinically proven case of otomycosis.
  21. Probiotics in the prevention of necrotizing enterocolitis in preterm infants. DBT Funded Multicentre, randomized, double-blind, comparative study to evaluate the efficacy and safety of intravenous ceftaroline fosamil versus intravenous ceftrizxone in the treatment of adult hospitalized patients with community-acquired bacterial pneumonia in Asia. AstraZeneca AB 151 85 Sodertalje, Sweden.